yepd agar  (Difco)

Journal: mBio

Article Title: Manipulation of regulators of morphogenesis is not sufficient to render a Candida albicans colonizer strain pathogenic

doi: 10.1128/mbio.00415-26

Figure Lengend Snippet: The Ras1-cAMP pathway promotes filamentation in intrinsically low-damage-inducing strains. ( A ) C. albicans strain 101 wild type and 101_ BRG1 FL / BRG1 FL were plated on Spider agar for 7 days at 35°C. ( B ) Colony morphology of clones that were isolated from spontaneously outgrown filaments ( A ) when grown on YEPD or Spider agar, as indicated. ( C and D ) ECE1 expression levels ( C ) and LDH release ( D ) by the clones after exposure to TR146 keratinocytes for 24 h at 37°C, 5% CO 2 . Each symbol represents one sample. The mean ± SD is indicated. Statistical significance was determined using one-way ANOVA comparing each mutant with the corresponding parental strain. * P < 0.05, ** P < 0.01; ns, non-significant ( P > 0.05) ( E ) Mutations in RAS1 identified in the clones shown in panel B .

Article Snippet: Filamentation was assessed on YEPD agar (yeast extract peptone dextrose [YEPD], 1% Bacto peptone [Difco Laboratories, Basel, Switzerland], 0.5% yeast extract [Difco], 2% glucose [Sigma], and 2% agar [Difco]) and YCB/BSA agar (1.17% yeast carbon base [Becton Dickinson] and 2% BSA [Sigma]).

Techniques: Clone Assay, Isolation, Expressing, Mutagenesis

Journal: bioRxiv

Article Title: On the mode of anti- C. albicans activity of a bis(benzoxaborole) analogue of Tavaborole

doi: 10.1101/2024.07.29.605614

Figure Lengend Snippet: Candida albicans metabolic activity reduction by benzoxaborole derivatives Influence of bezoxaboroles concentrations on metabolic activity of blastoconidial cells. MTS test (3- (4.5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, Promega, USA) was used to assess C. albicans proliferation. ( A ) The suspension of C. albicans cells in medium YEPD at the density of 1-3 x 10 2 CFU/mL was incubated with compounds 2 and Tavaborole at the range of conc.: 0.25-256 μg/mL for 48 h at 37 ° C. ( B ) The activity of benzoxaboroles against C. albicans growing in a medium with exogenous ergosterol (exErg) at 400 µg/mL for 48 h. %viable cells = OD490 test sample/OD490 control x 100%, control means cells treated with 0.96% DMSO. The results from 3 independent experiments were analyzed. Statistically significant differences were noted between non-treated growth and treated samples (P< 0.05, Unpaired t-test).

Article Snippet: [ ] Appropriately, after 10-fold dilution series 100 μL aliquots from microtiter plates were used to inoculate YEPD agar plates (A&A Biotechnology, Poland) and after 48h incubation at 37°C the grown colonies were counted.

Techniques: Activity Assay, Suspension, Incubation, Control

Journal: Cell Genomics

Article Title: Non-additive genetic components contribute significantly to population-wide gene expression variation

doi: 10.1016/j.xgen.2023.100459

Figure Lengend Snippet:

Article Snippet: YPD (YEPD) Agar (Powder) , MP Biomedicals , 4001232.

Techniques: Recombinant, RNA Sequencing Assay, Software